sodium citrate buffer (Vector Laboratories)

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Vector Laboratories sodium citrate buffer
Sodium Citrate Buffer, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2632 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antigens/Antigen+Unmasking+Solution%2C+Citric+Acid+Based/pm42867991-661-9-12
Average 96 stars, based on 2632 article reviews

sodium citrate buffer - by Bioz Stars, 2026-11

96/100 stars

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Article Title: Knockdown of microRNA-214-3p Promotes Tumor Growth and Epithelial-Mesenchymal Transition in Prostate Cancer
Article Snippet: For IHC staining, deparaffinized sections were pretreated to retrieve antigens with a Tris-based Antigen Unmasking Solution (Vector Laboratories, Burlingame, CA, USA) before blocking with 10% normal serum and then applying either of the previously discussed antibodies and anti-PTK6 (Thermo Fisher Scientific) at 4 °C overnight.

Article Title: Induction of Fatty Acid Oxidation Underlies DNA Damage-Induced Cell Death and Ameliorates Obesity-Driven Chemoresistance.
Article Snippet: Antigens were revealed by DAB (Vector Laboratories, SK-4100) and then the slides were counterstained with EASYSTAIN Harris Hematocylin (YD diagnostics, S2-5).

Avidin-Biotin Assay:

Article Title: Role of Angiopoietin-2 in Regulating Growth and Vascularity of Astrocytomas
Article Snippet: Primary antibodies used include: FactorVIII (rabbit polyclonal antibody #A0082; DAKO; used at 1 : 2500) and a polyclonal goat anti-Ang2 antibody (1 : 200 and 1 : 400, Santa Cruz). .. Secondary antibody was a goat antimouse antibody (Zymed) used at 1 : 200, and antigens were detected using the avidin-biotin complex method (Vector Laboratories) and diaminobenzidine substrate. .. All analyses were completed using StatView 4.1 for the Macintosh (Abacus Concepts, Berkeley, CA).

Immunohistochemistry:

Article Title: Alcohol drinking inhibits NOTCH–PAX9 signaling in esophageal squamous epithelial cells
Article Snippet: Proteins were detected with a rabbit monoclonal anti-cleaved NOTCH1 antibody (1:500, Cat #: 4147, Cell Signaling Technology, Danvers, MA, USA), a rabbit monoclonal anti-PAX9 antibody (1:600, Cat #: 12847, Cell Signaling Technology), a rabbit monoclonal anti-HES1 antibody (1:4000, Cat #: 11988, Cell Signaling Technology), a rabbit monoclonal anti-RBPJ antibody (1:2000, Cat #: 5313, Cell Signaling Technology), a rabbit polyclonal anti-c-MYC antibody (1:1000, Cat #: 9402, Cell Signaling Technology), a mouse monoclonal anti-STAT3 antibody (1:1000, Cat #: 9139, Cell Signaling Technology), a rabbit polyclonal anti-p-STAT3 antibody (1:1000, Cat #: 9131, Cell Signaling Technology), a rabbit polyclonal anti-SOX2 antibody (1:2000, Cat #: ab97959, Abcam, Cambridge, MA, USA), a rabbit polyclonal anti-ETV4 antibody (1:1000, Cat #: ab135590, Abcam) and a mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:40000, Cat #: ab8245, Abcam). .. For IHC staining, deparaffinized sections were pretreated to retrieve antigens with a Tris-based Antigen Unmasking Solution (Vector Laboratories, Burlingame, CA, USA), before blocking with 10% normal serum and then applying either a rabbit monoclonal anti-NOTCH1 antibody (1:200, Cat #: D1E11, Cell Signaling Technology), a rabbit polyclonal anti-NOTCH2 antibody (1:100, Cat #: ab52302, Abcam), a rabbit polyclonal anti-activated NOTCH1 antibody (1:100, Cat #: ab8925, Abcam), a rabbit monoclonal anti-PAX9 antibody (1:50, Cell Signaling Technology) or a rabbit monoclonal anti-HES1 antibody (1:50, Cell Signaling Technology) at 4°C for overnight. ..

Article Title: Alcohol drinking inhibits NOTCH–PAX9 signaling in esophageal squamous epithelial cells
Article Snippet: Proteins were detected with a rabbit monoclonal anti-cleaved NOTCH1 antibody (1:500, Cat #: 4147, Cell Signaling Technology, Danvers, MA, USA), a rabbit monoclonal anti-PAX9 antibody (1:600, Cat #: 12847, Cell Signaling Technology), a rabbit monoclonal anti-HES1 antibody (1:4000, Cat #: 11988, Cell Signaling Technology), a rabbit monoclonal anti-RBPJ antibody (1:2000, Cat #: 5313, Cell Signaling Technology), a rabbit polyclonal anti-c-MYC antibody (1:1000, Cat #: 9402, Cell Signaling Technology), a mouse monoclonal anti-STAT3 antibody (1:1000, Cat #: 9139, Cell Signaling Technology), a rabbit polyclonal anti-p-STAT3 antibody (1:1000, Cat #: 9131, Cell Signaling Technology), a rabbit polyclonal anti-SOX2 antibody (1:2000, Cat #: ab97959, Abcam, Cambridge, MA, USA), a rabbit polyclonal anti-ETV4 antibody (1:1000, Cat #: ab135590, Abcam) and a mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:40000, Cat #: ab8245, Abcam). .. Immunohistochemical (IHC) staining For IHC staining, deparaffinized sections were pretreated to retrieve antigens with a Tris-based Antigen Unmasking Solution (Vector Laboratories, Burlingame, CA, USA), before blocking with 10% normal serum and then applying either a rabbit monoclonal anti-NOTCH1 antibody (1:200, Cat #: D1E11, Cell Signaling Technology), a rabbit polyclonal anti-NOTCH2 antibody (1:100, Cat #: ab52302, Abcam), a rabbit polyclonal anti-activated NOTCH1 antibody (1:100, Cat #: ab8925, Abcam), a rabbit monoclonal anti-PAX9 antibody (1:50, Cell Signaling Technology) or a rabbit monoclonal anti-HES1 antibody (1:50, Cell Signaling Technology) at 4°C for overnight. ..

Staining:

Article Title: Alcohol drinking inhibits NOTCH–PAX9 signaling in esophageal squamous epithelial cells
Article Snippet: Proteins were detected with a rabbit monoclonal anti-cleaved NOTCH1 antibody (1:500, Cat #: 4147, Cell Signaling Technology, Danvers, MA, USA), a rabbit monoclonal anti-PAX9 antibody (1:600, Cat #: 12847, Cell Signaling Technology), a rabbit monoclonal anti-HES1 antibody (1:4000, Cat #: 11988, Cell Signaling Technology), a rabbit monoclonal anti-RBPJ antibody (1:2000, Cat #: 5313, Cell Signaling Technology), a rabbit polyclonal anti-c-MYC antibody (1:1000, Cat #: 9402, Cell Signaling Technology), a mouse monoclonal anti-STAT3 antibody (1:1000, Cat #: 9139, Cell Signaling Technology), a rabbit polyclonal anti-p-STAT3 antibody (1:1000, Cat #: 9131, Cell Signaling Technology), a rabbit polyclonal anti-SOX2 antibody (1:2000, Cat #: ab97959, Abcam, Cambridge, MA, USA), a rabbit polyclonal anti-ETV4 antibody (1:1000, Cat #: ab135590, Abcam) and a mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:40000, Cat #: ab8245, Abcam). .. Immunohistochemical (IHC) staining For IHC staining, deparaffinized sections were pretreated to retrieve antigens with a Tris-based Antigen Unmasking Solution (Vector Laboratories, Burlingame, CA, USA), before blocking with 10% normal serum and then applying either a rabbit monoclonal anti-NOTCH1 antibody (1:200, Cat #: D1E11, Cell Signaling Technology), a rabbit polyclonal anti-NOTCH2 antibody (1:100, Cat #: ab52302, Abcam), a rabbit polyclonal anti-activated NOTCH1 antibody (1:100, Cat #: ab8925, Abcam), a rabbit monoclonal anti-PAX9 antibody (1:50, Cell Signaling Technology) or a rabbit monoclonal anti-HES1 antibody (1:50, Cell Signaling Technology) at 4°C for overnight. ..

Blocking Assay:

Article Title: Alcohol drinking inhibits NOTCH–PAX9 signaling in esophageal squamous epithelial cells
Article Snippet: Proteins were detected with a rabbit monoclonal anti-cleaved NOTCH1 antibody (1:500, Cat #: 4147, Cell Signaling Technology, Danvers, MA, USA), a rabbit monoclonal anti-PAX9 antibody (1:600, Cat #: 12847, Cell Signaling Technology), a rabbit monoclonal anti-HES1 antibody (1:4000, Cat #: 11988, Cell Signaling Technology), a rabbit monoclonal anti-RBPJ antibody (1:2000, Cat #: 5313, Cell Signaling Technology), a rabbit polyclonal anti-c-MYC antibody (1:1000, Cat #: 9402, Cell Signaling Technology), a mouse monoclonal anti-STAT3 antibody (1:1000, Cat #: 9139, Cell Signaling Technology), a rabbit polyclonal anti-p-STAT3 antibody (1:1000, Cat #: 9131, Cell Signaling Technology), a rabbit polyclonal anti-SOX2 antibody (1:2000, Cat #: ab97959, Abcam, Cambridge, MA, USA), a rabbit polyclonal anti-ETV4 antibody (1:1000, Cat #: ab135590, Abcam) and a mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:40000, Cat #: ab8245, Abcam). .. Immunohistochemical (IHC) staining For IHC staining, deparaffinized sections were pretreated to retrieve antigens with a Tris-based Antigen Unmasking Solution (Vector Laboratories, Burlingame, CA, USA), before blocking with 10% normal serum and then applying either a rabbit monoclonal anti-NOTCH1 antibody (1:200, Cat #: D1E11, Cell Signaling Technology), a rabbit polyclonal anti-NOTCH2 antibody (1:100, Cat #: ab52302, Abcam), a rabbit polyclonal anti-activated NOTCH1 antibody (1:100, Cat #: ab8925, Abcam), a rabbit monoclonal anti-PAX9 antibody (1:50, Cell Signaling Technology) or a rabbit monoclonal anti-HES1 antibody (1:50, Cell Signaling Technology) at 4°C for overnight. ..

Article Title: Transmembrane Mucin 1 Blocks Fluorescein Ingress to Corneal Epithelium
Article Snippet: .. After antigens were retrieved by a Tris-Based antigen unmasking solution (no. H-3301; Vector Laboratories, Burlingame, CA, USA) for one minute, a BLOXALL Endogenous Blocking Solution (no. SP-6000; Vector Laboratories) was used to inactivate endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase. .. A Streptavidin/Biotin Blocking Kit (no. SP-2002; Vector Laboratories) was used to block all endogenous biotin, biotin receptors, and streptavidin binding sites.

Immunohistochemical staining:

Article Title: Alcohol drinking inhibits NOTCH–PAX9 signaling in esophageal squamous epithelial cells
Article Snippet: Proteins were detected with a rabbit monoclonal anti-cleaved NOTCH1 antibody (1:500, Cat #: 4147, Cell Signaling Technology, Danvers, MA, USA), a rabbit monoclonal anti-PAX9 antibody (1:600, Cat #: 12847, Cell Signaling Technology), a rabbit monoclonal anti-HES1 antibody (1:4000, Cat #: 11988, Cell Signaling Technology), a rabbit monoclonal anti-RBPJ antibody (1:2000, Cat #: 5313, Cell Signaling Technology), a rabbit polyclonal anti-c-MYC antibody (1:1000, Cat #: 9402, Cell Signaling Technology), a mouse monoclonal anti-STAT3 antibody (1:1000, Cat #: 9139, Cell Signaling Technology), a rabbit polyclonal anti-p-STAT3 antibody (1:1000, Cat #: 9131, Cell Signaling Technology), a rabbit polyclonal anti-SOX2 antibody (1:2000, Cat #: ab97959, Abcam, Cambridge, MA, USA), a rabbit polyclonal anti-ETV4 antibody (1:1000, Cat #: ab135590, Abcam) and a mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:40000, Cat #: ab8245, Abcam). .. Immunohistochemical (IHC) staining For IHC staining, deparaffinized sections were pretreated to retrieve antigens with a Tris-based Antigen Unmasking Solution (Vector Laboratories, Burlingame, CA, USA), before blocking with 10% normal serum and then applying either a rabbit monoclonal anti-NOTCH1 antibody (1:200, Cat #: D1E11, Cell Signaling Technology), a rabbit polyclonal anti-NOTCH2 antibody (1:100, Cat #: ab52302, Abcam), a rabbit polyclonal anti-activated NOTCH1 antibody (1:100, Cat #: ab8925, Abcam), a rabbit monoclonal anti-PAX9 antibody (1:50, Cell Signaling Technology) or a rabbit monoclonal anti-HES1 antibody (1:50, Cell Signaling Technology) at 4°C for overnight. ..

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